Description
The Intrinsic Hepcidin IDx™ ELISA kit is a monoclonal antibody-based assay that binds with high affinity to the N-terminus of hepcidin-25. This antibody also binds low abundance, N-terminus isomers of hepcidin-25 with lower affinities.
Hepcidin is a 25-amino acid peptide hormone that regulates dietary iron absorption from the duodenum, controls recycling of senescent erythrocyte iron by macrophages, and manages iron transport from hepatocytes into plasma. Hepcidin is positively regulated by plasma iron and IL-6 (inflammation, infection) and is suppressed by erythropoiesis via erythroferrone. Abnormally low serum hepcidin is associated with iron deficiency anemia (IDA) and hereditary hemochromatosis, and high serum hepcidin can lead to iron sequestration and to anemia of inflammation (anemia of chronic disease) observed in chronic kidney disease (CKD), rheumatoid arthritis (RA), various cancers, and iron refractory iron deficiency anemia (IRIDA). Hepcidin levels have been shown to be useful in predicting response to oral iron therapy.
U.S. Pat. No. 9,657,098; NZ Pat. No. 631098; Pat. Pending in the US and abroad.
ICE-007 Product Insert v10
Researcher’s Guide to Hepcidin Testing
Questions? Please email us: [email protected]
General Information
| Catalog number |
ICE-007 |
| Quantity |
96 Wells |
| Application |
ELISA, EIA, RUO |
| Method |
Competitive |
| Antibody type |
Monoclonal |
| Target species |
Human |
| Analyte |
Hepcidin-25, HAMP, HEPC, LEAP1 |
| Assay time |
1.75 hr |
| Detection method |
Colormetric |
| Sample type |
Serum |
| Sample volume required per well |
20 ul |
| Detection range |
5 – 250 ng/ML |
| Sensitivity |
2.5 ng/mL |
| Regulatory Status |
Research use only ELISA |
| Storage requirements |
2-8 c |
Typical data
| Hepcidin-25 Standard |
Concentration (ng/ML) |
Absorbance (450nm) |
| 1 |
0 |
2.36 |
| 2 |
2.5 |
2.14 |
| 3 |
10 |
1.61 |
| 4 |
25 |
0.90 |
| 5 |
50 |
0.47 |
| 6 |
100 |
0.25 |
| 7 |
250 |
0.10 |
| 8 |
1000 |
0.03 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
16 |
16 |
16 |
16 |
16 |
16 |
| Mean(ng/ml) |
21.8 |
92.8 |
219.9 |
22.2 |
92.8 |
222.5 |
| Standard deviation |
0.68 |
2.22 |
8.89 |
0.73 |
24.1 |
8.56 |
| CV (%) |
3.1 |
2.4 |
4.0 |
3.3 |
2.6 |
3.8 |
Linearity
|
Serum |
| 1:2 |
Range (%) |
96-99 |
|
Average (%) |
97 |
| 1:4 |
Range (%) |
92-101 |
|
Average (%) |
98 |
Recovery
| Sample Type |
Range (%) |
Average Recovery (%) |
| Serum |
91-109 |
97 |
