Description
The Intrinsic LifeSciences Hepcidin-Murine Compete™ ELISA is designed for quantification of hepcidin-1 (hepc-1) in murine serum and urine. Murine hepcidin-2 (hepc-2) is not detected and does not interfere with hepc-1 measurements using this ELISA. This kit does not contain mercaptoethanol, azide- or mercury-based preservatives.
Hepcidin was discovered to be an antimicrobial peptide produced in the liver and was identified as the peptide hormone that regulates extracellular iron in response to changes in dietary and systemic iron load, anemia, hypoxia, erythropoiesis, and inflammation. Hepcidin inhibits cellular iron efflux by binding to and inducing degradation of the sole known iron channel, ferroportin. Synthetic hepcidin injected into mice binds to ferroportin-rich tissues and rapidly lowers serum iron levels. Data on the normal ranges of circulating levels of the hepcidin-1 protein in common mouse model strains (e.g. Bl/6) are not available currently. Murine hepc-1 peptide levels will scale closely with dietary iron concentration of the feed and will correlate with hepc-1 gene expression measured by qPCR.
HMC-001 Product Insert
Researcher’s Guide to Hepcidin Testing
Questions? Please email us: [email protected]
General Information
| Catalog number |
HMC-001 |
| Quantity |
96 Wells |
| Application |
ELISA, EIA, RUO |
| Method |
Competitive |
| Antibody type |
Monoclonal |
| Target species |
Mouse |
| Analyte |
Hepcidin-1, HAMP1, HEPC1 |
| Assay time |
2h 45m |
| Detection method |
Colormetric |
| Sample type |
Serum |
| Sample volume required per well |
12 ul |
| Detection range |
0.5-333 ng/ml |
| Sensitivity |
0.18 ng/ml |
| Regulatory Status |
Research Use Only ELISA |
| Storage requirements |
2-8 c |
Typical data
| Hepcidin-25 Standard |
Concentration (ng/ML) |
Absorbance (450nm) |
| 1 |
1000 |
0.065 |
| 2 |
333.3 |
0.078 |
| 3 |
111.1 |
0.115 |
| 4 |
37.0 |
0.212 |
| 5 |
12.3 |
0.422 |
| 6 |
4.1 |
0.765 |
| 7 |
1.4 |
1.106 |
| 8 |
0 |
1.457 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
16 |
16 |
16 |
16 |
16 |
16 |
| Mean(ng/ml) |
25.2 |
80.8 |
202.7 |
24.5 |
79.0 |
204.0 |
| Standard deviation |
1.30 |
2.26 |
7.15 |
1.08 |
1.64 |
3.04 |
| CV (%) |
5.2 |
2.8 |
3.5 |
4.4 |
2.1 |
1.5 |
Linearity
|
Serum |
| 1:4 |
Range (%) |
90-102% |
|
Average (%) |
95% |
| 1:8 |
Range (%) |
90-102% |
|
Average (%) |
95% |
| 1:16 |
Range (%) |
85-99% |
|
Average (%) |
92% |
Recovery
| Sample Type |
Range (%) |
Average Recovery (%) |
| Serum |
93-107 |
100 |
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