Description
The Intrinsic LifeSciences Primate-Hepcidin CompeteTM competitive ELISA is designed for quantification of hepcidin-25 in baboon or cynomolgus monkey serum. Quantification of hepcidin-25 from other primate species or from plasma or other body fluids has not been evaluated. This kit does not contain mercaptoethanol, azide- or mercury-based preservatives.
Hepcidin was discovered to be an antimicrobial peptide produced in the liver and is the peptide hormone that regulates extracellular iron in response to changes in dietary and systemic iron load. Hepcidin also regulates iron in response to anemia, hypoxia, erythropoiesis, and inflammation. Hepcidin is an acute phase protein that is elevated in anemia of inflammation. IL-6 is a principal regulator of hepcidin during inflammation. Hepcidin inhibits cellular iron efflux by binding to and inducing degradation of the sole known iron channel, ferroportin. Hepcidin is the master regulator of iron homeostasis in all vertebrates.
U.S. Pat. No. 9,657,098; NZ Pat. No. 631098; Pat. Pending in the US and abroad
PHC-001 Product Insert
Researcher’s Guide to Hepcidin Testing
Questions? Please email us: [email protected]
General Information
| Catalog number |
PHC-001 |
| Quantity |
96 Wells |
| Application |
ELISA, EIA, RUO |
| Method |
Competative |
| Antibody type |
Monoclonal |
| Target species |
Baboon, Cynomolgus |
| Analyte |
Hepcidin-25, HAMP, HEPC, LEAP1 |
| Assay time |
1hr 40m |
| Detection method |
Colormetric |
| Sample type |
Serum |
| Sample volume required per well |
24 ul |
| Detection range |
1 – 250 ng/ML |
| Sensitivity |
0.5 ng/mL |
| Regulatory Status |
Research use only. Not for use in diagnostic procedures. |
| Storage requirements |
2-8 c |
Typical data
| Hepcidin-25 Standard |
Concentration (ng/ML) |
Absorbance (450nm) |
| 1 |
400 |
0.0648 |
| 2 |
133.3 |
0.1043 |
| 3 |
44.4 |
0.2168 |
| 4 |
14.8 |
0.5247 |
| 5 |
4.9 |
1.0188 |
| 6 |
1.65 |
1.5767 |
| 7 |
0.55 |
1.7773 |
| 8 |
0.0001 |
1.888 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
16 |
16 |
16 |
16 |
16 |
16 |
| Mean(ng/ml) |
16.9 |
93.1 |
139.0 |
17.6 |
89.3 |
134.0 |
| Standard deviation |
2.14 |
2.03 |
6.92 |
1.02 |
5.39 |
6.98 |
| CV (%) |
12.7 |
2.2 |
5.0 |
5.8 |
6.0 |
5.2 |
Linearity
|
Serum |
| 1:2 |
Range (%) |
96-99 |
|
Average (%) |
97 |
| 1:4 |
Range (%) |
92-1015 |
|
Average (%) |
98 |
Recovery
| Sample Type |
Range (%) |
Average Recovery (%) |
| Serum |
85-113 |
98 |
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